PRIME-XS :: File Repository

04 - Protocols

Protocols used at the Transnational Access sites of the PRIME-XS project.

Files

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PRIME-XS Protocol ERLIC for isocratic separation of charged solutes and selective isolation of phosphopeptides.pdf

Short Description:
Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) for isocratic separation of charged solutes and selective isolation of phosp...
Submitted On:
07 Feb 2014
File Size:
527.14 Kb
Downloads:
172

PRIME-XS Protocol NPC Bradford Protein Assay.pdf

Short Description:
PRIME-XS protocol for the determination of protein concentration using the Bradford Assay.
Submitted On:
18 Oct 2013
File Size:
97.75 Kb
Downloads:
267

PRIME-XS Protocol NPC Cell Lysis.pdf

Short Description:
PRIME-XS protocol for the lysis of cells and tissue sample, compatible with LC-MS/MS analysis
Submitted On:
18 Oct 2013
File Size:
120.48 Kb
Downloads:
343

PRIME-XS Protocol NPC HILIC peptide fractionation

Short Description:
PRIME-XS Protocol NPC HILIC peptide fractionation
Submitted On:
12 Aug 2013
File Size:
615.29 Kb
Downloads:
129

PRIME-XS Protocol NPC In Gel Digestion.pdf

Short Description:
PRIME-XS protocol for in gel digestion of proteins using trypsin from Coomassie or Silver stained gels.
Submitted On:
18 Oct 2013
File Size:
109.55 Kb
Downloads:
275

PRIME-XS Protocol NPC In Solution Digestion.pdf

Short Description:
PRIME-XS protocol for the in-solution digestion of proteins using various different enzymes (Trypsin, Lys-C, Lys-N).
Submitted On:
18 Oct 2013
File Size:
145.32 Kb
Downloads:
450

PRIME-XS Protocol NPC Lys-N Digestion.pdf

Short Description:
PRIME-XS protocol for the in-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N.
Submitted On:
18 Oct 2013
File Size:
119.42 Kb
Downloads:
175
File Author:
Reinout Raijmakers

PRIME-XS Protocol NPC Peptide Dimethyl Labeling.pdf

Short Description:
PRIME-XS protocol for triple dimethyl labeling of peptides in solution for quantitative proteomics analysis of multiple samples. Note: this protoco...
Submitted On:
02 May 2014
File Size:
334.86 Kb
Downloads:
303

PRIME-XS Protocol SILAC Bacteria.pdf

Short Description:
PRIME-XS protocol for the Stable Isotope Labeling by Amino Acids Applied of Bacterial Cell Cultures
Submitted On:
02 May 2014
File Size:
483.86 Kb
Downloads:
106

PRIME-XS Protocol TiO2 phosphopeptide enrichment

Short Description:
PRIME-XS Protocol for the enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis.
Submitted On:
18 Oct 2013
File Size:
632.94 Kb
Downloads:
546
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