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04 - Protocols
Protocols used at the Transnational Access sites of the PRIME-XS project.
PRIME-XS Protocol ERLIC for isocratic separation of charged solutes and selective isolation of phosphopeptides.pdf
- Short Description:
- Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) for isocratic separation of charged solutes and selective isolation of phosp...
- Submitted On:
- 07 Feb 2014
- File Size:
- 527.14 Kb
- Downloads:
- 182
PRIME-XS Protocol NPC Bradford Protein Assay.pdf
- Short Description:
- PRIME-XS protocol for the determination of protein concentration using the Bradford Assay.
- Submitted On:
- 18 Oct 2013
- File Size:
- 97.75 Kb
- Downloads:
- 281
PRIME-XS Protocol NPC Cell Lysis.pdf
- Short Description:
- PRIME-XS protocol for the lysis of cells and tissue sample, compatible with LC-MS/MS analysis
- Submitted On:
- 18 Oct 2013
- File Size:
- 120.48 Kb
- Downloads:
- 357
PRIME-XS Protocol NPC HILIC peptide fractionation
- Short Description:
- PRIME-XS Protocol NPC HILIC peptide fractionation
- Submitted On:
- 12 Aug 2013
- File Size:
- 615.29 Kb
- Downloads:
- 140
PRIME-XS Protocol NPC In Gel Digestion.pdf
- Short Description:
- PRIME-XS protocol for in gel digestion of proteins using trypsin from Coomassie or Silver stained gels.
- Submitted On:
- 18 Oct 2013
- File Size:
- 109.55 Kb
- Downloads:
- 295
PRIME-XS Protocol NPC In Solution Digestion.pdf
- Short Description:
- PRIME-XS protocol for the in-solution digestion of proteins using various different enzymes (Trypsin, Lys-C, Lys-N).
- Submitted On:
- 18 Oct 2013
- File Size:
- 145.32 Kb
- Downloads:
- 469
PRIME-XS Protocol NPC Lys-N Digestion.pdf
- Short Description:
- PRIME-XS protocol for the in-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N.
- Submitted On:
- 18 Oct 2013
- File Size:
- 119.42 Kb
- Downloads:
- 183
- File Author:
- Reinout Raijmakers
PRIME-XS Protocol NPC Peptide Dimethyl Labeling.pdf
- Short Description:
- PRIME-XS protocol for triple dimethyl labeling of peptides in solution for quantitative proteomics analysis of multiple samples. Note: this protoco...
- Submitted On:
- 02 May 2014
- File Size:
- 334.86 Kb
- Downloads:
- 316
PRIME-XS Protocol SILAC Bacteria.pdf
- Short Description:
- PRIME-XS protocol for the Stable Isotope Labeling by Amino Acids Applied of Bacterial Cell Cultures
- Submitted On:
- 02 May 2014
- File Size:
- 483.86 Kb
- Downloads:
- 115
PRIME-XS Protocol TiO2 phosphopeptide enrichment
- Short Description:
- PRIME-XS Protocol for the enrichment of phosphopeptides using titanium dioxide (TiO2) affinity chromatography, for LC-MS/MS analysis.
- Submitted On:
- 18 Oct 2013
- File Size:
- 632.94 Kb
- Downloads:
- 591
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