PRIME-XS :: File Repository

04 - Protocols

Protocols used at the Transnational Access sites of the PRIME-XS project.

Files

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PRIME-XS Protocol UCAM Affinity Purification MS.pdf

Short Description:
PRIME-XS Protocol for the mass spectrometric characterization of protein-protein interactions using antibody affinity isolation of GFP tagged prot...
Submitted On:
18 Oct 2013
File Size:
248.43 Kb
Downloads:
79

PRIME-XS Protocol UCAM LOPIT.pdf

Short Description:
PRIME-XS protocol for the localisation of organelle proteins using isotope tagging (LOPIT). The output of a LOPIT experiment is the steady location...
Submitted On:
18 Oct 2013
File Size:
333.61 Kb
Downloads:
79

PRIME-XS Protocol UCAM Spatial Proteomics Analysis.pdf

Short Description:
PRIME-XS protocol for organelle proteomics data analysis: Application of statistical machine learning to infer protein sub-cellular localisation us...
Submitted On:
18 Oct 2013
File Size:
168.69 Kb
Downloads:
41

PRIME-XS Protocol UZH Affinity Purification and Selective Reaction Monitoring (SRM)

Short Description:
PRIME-XS Protocol for Affinity Purification and Selective Reaction Monitoring (SRM)
Submitted On:
18 Oct 2013
File Size:
203.17 Kb
Downloads:
77

PRIME-XS Protocol VIB Enrichment of peptides containing methionine sulfoxide by COFRADIC

Short Description:
Oxidation of methionine residues to methionine sulfoxides was found following oxidative stress. This is a PRIME-XS protocol for COFRADIC for enri...
Submitted On:
18 Oct 2013
File Size:
156.13 Kb
Downloads:
70

PRIME-XS Protocol VIB Enrichment of peptides containing nitrated tyrosine residues by COFRADIC

Short Description:
Nitration of tyrosine residues readily occurs when cells are under conditions of oxidative or nitrosative stress. This PRIME-XS protocol is based o...
Submitted On:
18 Oct 2013
File Size:
135.14 Kb
Downloads:
52

PRIME-XS Protocol VIB Enrichment of tryptic N-terminal peptides by COFRADIC

Short Description:
This PRIME-XS protocol is used for enriching N-terminal peptides from whole proteome digests using the positional proteomics N-terminal COFRADIC te...
Submitted On:
18 Oct 2013
File Size:
155.95 Kb
Downloads:
71

PRIME-XS protocol VIB Enrichment of tryptic N-terminal peptides through SCX

Short Description:
This is a PRIME-XS protocol for strong cation exchange chromatography at acidic pH is to enrich for protein N-terminal peptides that are proxies fo...
Submitted On:
18 Oct 2013
File Size:
132.00 Kb
Downloads:
76

PRIME-XS Protocol VIB In-Gel Stable Isotope Labeling (ISIL)

Short Description:
This PRIME-XS protocol is used to mass tag primary amines of gel-separated proteins. This mass tagging occurs also at protein N-termini (alpha-amin...
Submitted On:
18 Oct 2013
File Size:
87.00 Kb
Downloads:
59

PRIME-XS Protocol VIB N-propionylation of endoLys-C generated peptides

Short Description:
This PRIME-XS protocol is used for post-metabolic labeling of peptides generated by endoproteinase Lys-C for differential proteome analysis.
Submitted On:
18 Oct 2013
File Size:
139.36 Kb
Downloads:
78
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