PRIME-XS :: File Repository

04 - Protocols

Protocols used at the Transnational Access sites of the PRIME-XS project.

Files

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PRIME-XS Protocol NPC Peptide Dimethyl Labeling.pdf

Short Description:
PRIME-XS protocol for triple dimethyl labeling of peptides in solution for quantitative proteomics analysis of multiple samples. Note: this protoco...
Submitted On:
02 May 2014
File Size:
334.86 Kb
Downloads:
303

PRIME-XS Protocol SILAC Bacteria.pdf

Short Description:
PRIME-XS protocol for the Stable Isotope Labeling by Amino Acids Applied of Bacterial Cell Cultures
Submitted On:
02 May 2014
File Size:
483.86 Kb
Downloads:
107

PRIME-XS Protocol ERLIC for isocratic separation of charged solutes and selective isolation of phosphopeptides.pdf

Short Description:
Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) for isocratic separation of charged solutes and selective isolation of phosp...
Submitted On:
07 Feb 2014
File Size:
527.14 Kb
Downloads:
173

PRIME-XS Protocol UCAM Spatial Proteomics Analysis.pdf

Short Description:
PRIME-XS protocol for organelle proteomics data analysis: Application of statistical machine learning to infer protein sub-cellular localisation us...
Submitted On:
18 Oct 2013
File Size:
168.69 Kb
Downloads:
41

PRIME-XS Protocol UCAM LOPIT.pdf

Short Description:
PRIME-XS protocol for the localisation of organelle proteins using isotope tagging (LOPIT). The output of a LOPIT experiment is the steady location...
Submitted On:
18 Oct 2013
File Size:
333.61 Kb
Downloads:
79

PRIME-XS Protocol UCAM Affinity Purification MS.pdf

Short Description:
PRIME-XS Protocol for the mass spectrometric characterization of protein-protein interactions using antibody affinity isolation of GFP tagged prot...
Submitted On:
18 Oct 2013
File Size:
248.43 Kb
Downloads:
79

PRIME-XS Protocol VIB N-propionylation of endoLys-C generated peptides

Short Description:
This PRIME-XS protocol is used for post-metabolic labeling of peptides generated by endoproteinase Lys-C for differential proteome analysis.
Submitted On:
18 Oct 2013
File Size:
139.36 Kb
Downloads:
78

PRIME-XS Protocol VIB In-Gel Stable Isotope Labeling (ISIL)

Short Description:
This PRIME-XS protocol is used to mass tag primary amines of gel-separated proteins. This mass tagging occurs also at protein N-termini (alpha-amin...
Submitted On:
18 Oct 2013
File Size:
87.00 Kb
Downloads:
59

PRIME-XS protocol VIB Enrichment of tryptic N-terminal peptides through SCX

Short Description:
This is a PRIME-XS protocol for strong cation exchange chromatography at acidic pH is to enrich for protein N-terminal peptides that are proxies fo...
Submitted On:
18 Oct 2013
File Size:
132.00 Kb
Downloads:
76

PRIME-XS Protocol NPC Bradford Protein Assay.pdf

Short Description:
PRIME-XS protocol for the determination of protein concentration using the Bradford Assay.
Submitted On:
18 Oct 2013
File Size:
97.75 Kb
Downloads:
270
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