PRIME-XS :: File Repository

04 - Protocols

Protocols used at the Transnational Access sites of the PRIME-XS project.

Files

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PRIME-XS Protocol NPC Bradford Protein Assay.pdf

Short Description:
PRIME-XS protocol for the determination of protein concentration using the Bradford Assay.
Submitted On:
18 Oct 2013
File Size:
97.75 Kb
Downloads:
270

PRIME-XS Protocol NPC In Gel Digestion.pdf

Short Description:
PRIME-XS protocol for in gel digestion of proteins using trypsin from Coomassie or Silver stained gels.
Submitted On:
18 Oct 2013
File Size:
109.55 Kb
Downloads:
277

PRIME-XS Protocol NPC Peptide Dimethyl Labeling.pdf

Short Description:
PRIME-XS protocol for triple dimethyl labeling of peptides in solution for quantitative proteomics analysis of multiple samples. Note: this protoco...
Submitted On:
02 May 2014
File Size:
334.86 Kb
Downloads:
303

PRIME-XS Protocol NPC Cell Lysis.pdf

Short Description:
PRIME-XS protocol for the lysis of cells and tissue sample, compatible with LC-MS/MS analysis
Submitted On:
18 Oct 2013
File Size:
120.48 Kb
Downloads:
344

PRIME-XS Protocol NPC In Solution Digestion.pdf

Short Description:
PRIME-XS protocol for the in-solution digestion of proteins using various different enzymes (Trypsin, Lys-C, Lys-N).
Submitted On:
18 Oct 2013
File Size:
145.32 Kb
Downloads:
453

PRIME-XS Protocol NPC Lys-N Digestion.pdf

Short Description:
PRIME-XS protocol for the in-solution and in-gel digestion of proteins using Metalloendopeptidase Lys-N.
Submitted On:
18 Oct 2013
File Size:
119.42 Kb
Downloads:
176
File Author:
Reinout Raijmakers

PRIME-XS Protocol VIB In-Gel Stable Isotope Labeling (ISIL)

Short Description:
This PRIME-XS protocol is used to mass tag primary amines of gel-separated proteins. This mass tagging occurs also at protein N-termini (alpha-amin...
Submitted On:
18 Oct 2013
File Size:
87.00 Kb
Downloads:
59

PRIME-XS Protocol VIB N-propionylation of endoLys-C generated peptides

Short Description:
This PRIME-XS protocol is used for post-metabolic labeling of peptides generated by endoproteinase Lys-C for differential proteome analysis.
Submitted On:
18 Oct 2013
File Size:
139.36 Kb
Downloads:
78

PRIME-XS Protocol VIB Enrichment of tryptic N-terminal peptides by COFRADIC

Short Description:
This PRIME-XS protocol is used for enriching N-terminal peptides from whole proteome digests using the positional proteomics N-terminal COFRADIC te...
Submitted On:
18 Oct 2013
File Size:
155.95 Kb
Downloads:
71

PRIME-XS Protocol VIB Enrichment of peptides containing nitrated tyrosine residues by COFRADIC

Short Description:
Nitration of tyrosine residues readily occurs when cells are under conditions of oxidative or nitrosative stress. This PRIME-XS protocol is based o...
Submitted On:
18 Oct 2013
File Size:
135.14 Kb
Downloads:
52
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